Table of Contents{1} The Physical Description of a protein {9} Computations from composition data {10} Aside: Volume concepts and the particle description {18} 3. DETERMINING THE MOLECULAR WEIGHT {24} ASIDE: Averaging and Weighting in Microscopic Measurements {31} Remember, Partial Specific Volume, ?p, cc/gram, {32} Since the molecular weight is ? Ni Mi, the molar volume of the protein, Vp, is simply {33} Hydration, ? , is difficult to measure: {34} Does hydration make a difference in protein volume? {35} Effective radius of the solvated protein: {38} Other ways of measuring particle effective radius: {42} Shape measure then is rexpt./rsphere {43} However, it does not work very well... {44} Comparing shape independent values with shape dependent values... {50} 5. SOLVATION (Amount & Location): {54} ASIDE: Relaxation Processes as Probes of Motion. {55} ASIDE: ...Organization decays similarly due to random motion. {57} ASIDE: ...Photoselection... |
Author: John E. Wampler
Email: wampler@bchiris.bmb.uga.edu Home Page: http://bmbiris.bmb.uga.edu/wampler Assignment: Garrett & Grisham, Sections 4.6, 5.1, 5.7, and Appendix to Chapter 5.Remember:
A useful source of reference material for detection of proteins in solution and in gels is the Molecular Probes catalog (see p. 180ff), Handbook of Fluorescent Probes and Research Chemicals, R. P. Haugland, Molecular Probes, Inc., Eugene, OR.References:
G. K. Ackers (1967) Molecular Sieve Studies of Interacting Protein Systems, J. Biol. Chem. 242, 3026-3034. |