CKII Substrates

Potential substrates of CKII were identifid by bioinformatic searches of the S. cerevisiae genome for proteins containing a good match to the CKII consensus phosphorylation site. Known substrates of CKII in S. cerevisiae are highlighted in bold. Several known substrates are not included on this list because the required match to the consensus is too stringent. Additional substrates are thus likely. (For details, see Glover III, C. V. 1998. On the physiological role of casein kinase II in Saccharomyces cerevisiae. Prog. Nucleic Acid Res. Mol. Biol. 59: 95-133).

Category

Protein | Function | CKII motif(s) | Location

 

Chromatin Structure

Hgh1 HMG1,2 homolog EEEEEEESEEEEDDDEEDE 0.96

DNA replication/repair

Tel2 telomere length regulation protein DCSDSDDEDENDE 0.61

Rad2 ss endonuclease, excision repair DFSEDEED 0.69

Srs2 3'-5' DNA helicase, UV repair DESEEDEEEDQE 0.58

Transcription

Rpa1 RNA pol I 190 kDa subunit EDTEDEMDGE 0.09

Rpb2 RNA pol II 140 kDa subunit EESEDDSE 0.13

Tfc4 TFIIIC 131kDa subunit (TPR motifs) EISEDDEED 0.08

Srp40 sup of AC40 subunit of RNA pol I and III SSSSSSSSSDESSSESESEDE 0.26

Rrn3 RNA pol I transcription initiation factor EDDDDLDDDSGDDDDE 0.44

Spt5 Spt4/5/6 transcription regulator EVSDDEDEDEDEED 0.18

Spt6 Spt4/5/6 transcription regulator FDSSEEDEDIDEDEDE 0.03 EDDEFSDEDDE 0.14

Ssl1 C2H2 zinc finger, sup of stem-loop protein SESEEDED 0.02

Rim1 C2H2 transcription factor (meiosis) EEVDDESEIEDD 0.89

Asf1 regulation of silent loci ENSDGDEEEGEEE 0.83

RNA processing/transport/degradation

Rrp3 pre-rRNA processing EESENEEEESEEEEEDDDED8 0.11

DGSDSDSDSETSSDDENDID 0.14

Nsr1 nucleolin homolog DSSSSSSDSSSDEEEEEE 0.21

Fpr3 immunophilin, nucleolar EDEIDEESSEEEEEE 0.19 AESESEDDEEDDDEDDE 0.25

Nmd2 nonsense-mediated mRNA decay DESEDEDDEDDEDD12 0.81

Pan1 poly-A tail exonuclease QESDDEDEDDEE 0.77

Protein synthesis

Sui2 eIF-2a SDSEDDEDESDDE-cooh 0.97

Tif5 eIF-5 AESDDDEEDDE-cooh 0.98

Rit1 initiator tRNA phophoribosyl transferase EDTDDEEE 0.75

Rpl7 ribosomal protein L30 EETEEEEDD 0.37

Ard1 N-terminal acetylation (with Nat1) EDSDEQDEND 0.24

Protein targeting

Npl1 preprotein translocation EYSTDDDETESDDESD 0.95

Pse1 protein secretion enhancer IESDDTDDEEE 0.31

Uso1 protein transport, ER to golgi EISSDEEDDEEDDEEDEEE 0.99

Sec7 traffic from golgi DESGSEEEEEEEEEEEEEE 0.08

Adb6 clathrin assembly protein (b-adaptin) EESSDEDEDESEESSDDDE 0.96

Srp1 nuclear import (importin/karyopherin a) ADSDEEDE 0.12

Nip1 nuclear import DDSDYDSSDEESDEEDG 0.12

Mft1 mitochondrial protein import DEDYSDEDEE 0.68

Protein degradation

Ubc8 E2 ubiquitin-conjugating enzyme (24 kDa) EEDSDSDEDMD 0.81

Cdc34 E2 ubiquitin-conjugating enzyme (34 kDa) EDESEDVEDVE 0.96

Ubr1 E3 ubiquitin-protein ligase (N-end recog.) DEDDSDDNDDSDE 0.99

Pim1d LON protease (mitochondrial matrix) EEESEELDD 0.31

Cell cycle

Cdc68 required for cyclin gene expression at Start DESEEGEDWDELE 0.98

Sic1 CDK inhibitor QESEDEED 0.71

Sap2 cell cycle-dependent association with Sit4 DELSDDSDEEYDNCEDED 0.95

Sap3 cell cycle-dependent association with Sit4 DNEISSDEEDSEDEDEEND 0.97

Clb3 cyclin (G2/M) EDDGESEEDEEEDQE 0.27

Mif2 centromere protein (CENP-C) DESEDEEE 0.59

Cbf2 centromere binding factor (chr. seg. & movt.) EETEDLDEEEE 0.11

Mad1 spindle assembly checkpoint NDSDDDDD 0.45

Pds1 metaphase-anaphase transition EESSDDDEGNEDSE 0.50

Mak16 nuclear events of cell cycle EENSQDEEED 0.65 SESDSESESDSDSDEE 0.83

Cell polarity/morphogenesis

Spa2 mating-induced morphogenesis (shmoo) DWSSEEEEEE 0.12 EEEESEDDDEEEEDEDFDVD 0.88

Metabolism

Gac1 regulates glycogen synthase, PP1 regulator DLYDSEDEDDIDE 0.20

Tps3 trehalose phosphate synthase/phosphatase EDTDSEDDIDSDLETD 0.23

Prp1 PRPP synthase VDSEDEEE 0.69

Channels/pumps

Pma2e plasma membrane proton pump DDSDSDEDID 0.07

Trk1e high affinity potassium transporter EDTDTEDDGNDEDDDEENE 0.82

Other

Pph22 protein phosphatase 2A subunit (essential) DEDTDEELED 0.11

Pas8 peroxisome biogenesis (Sec18 family, ATP) EENDESDDEDE 0.36

Son1 nuclear integrity EFDSDDDDISDDEEDEIEE 0.41

Prp20 RCC1 homolog (reg. chr. cond.) DADDSSDDEDGD 0.28

Leo1 weak similarity to Npl6 SDSDSDEDDEGE 0.18

Tsm1 essential DEEEEEGESEEEEEGEEE 0.23